کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10536953 962647 2010 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A broad glass transition in hydrated proteins
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
A broad glass transition in hydrated proteins
چکیده انگلیسی
We performed Raman and Brillouin scattering measurements to estimate glass transition temperature, Tg, of hydrated protein. The measurements reveal very broad glass transition in hydrated lysozyme with approximate Tg ∼ 180 ± 15 K. This result agrees with a broad range of Tg ∼ 160-200 K reported in literature for hydrated globular proteins and stresses the difference between behavior of hydrated biomolecules and simple glass-forming systems. Moreover, the main structural relaxation of the hydrated protein system that freezes at Tg ∼ 180 K remains unknown. We emphasize the difference between the “dynamic transition”, known as a sharp rise in mean-squared atomic displacement at temperatures around TD ∼ 200-230 K, and the glass transition. They have different physical origin and should not be confused.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics - Volume 1804, Issue 1, January 2010, Pages 15-19
نویسندگان
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