کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10536968 | 962647 | 2010 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cell wall proteome of wheat roots under flooding stress using gel-based and LC MS/MS-based proteomics approaches
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کلمات کلیدی
nanoLC-MS/MSG6PDHPMSFIEFCBB2-DECWPPVPPDTTCoomassie Brilliant Blue - Coomassie درخشان آبیLC-MS/MS - LC-MS / MSFlooding stress - استرس سیلابtwo-dimensional polyacrylamide gel electrophoresis - الکتروفورز ژل پلی آکریل آمید دو بعدیisoelectric focusing - تمرکز ذره ای الکتریکیMolecular mass - توده مولکولیCell wall - دیوارهٔ یاختهdithiothreitol - دیتیوتریتولphenylmethylsulfonyl fluoride - فنیل متیل سولفونیل فلورایدIsoelectric point - نقطه ایزوالکتریکProteomics - پروتئومیکسcell wall protein - پروتئین دیواره سلولیpolyvinyl polypyrrolidone - پلی وینیل پلیپیرلیدونCHAPS - چاپسglucose-6-phosphate dehydrogenase - گلوکز 6-فسفات دهیدروژنازWheat - گندم
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
Cell wall proteins (CWPs) are important both for maintenance of cell structure and for responses to abiotic and biotic stresses. In this study, a destructive CWP purification procedure was adopted using wheat seedling roots and the purity of the CWP extract was confirmed by minimizing the activity of glucose-6-phosphate dehydrogenase, a cytoplasmic marker enzyme. To determine differentially expressed CWPs under flooding stress, gel-based proteomic and LC-MS/MS-based proteomic techniques were applied. Eighteen proteins were found to be significantly regulated in response to flood by gel-based proteomics and 15 proteins by LC MS/MS-based proteomics. Among the flooding down-regulated proteins, most were related to the glycolysis pathway and cell wall structure and modification. However, the most highly up-regulated proteins in response to flooding belong to the category of defense and disease response proteins. Among these differentially expressed proteins, only methionine synthase, β-1,3-glucanases, and β-glucosidase were consistently identified by both techniques. The down-regulation of these three proteins suggested that wheat seedlings respond to flooding stress by restricting cell growth to avoid energy consumption; by coordinating methionine assimilation and cell wall hydrolysis, CWPs played critical roles in flooding responsiveness.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics - Volume 1804, Issue 1, January 2010, Pages 124-136
Journal: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics - Volume 1804, Issue 1, January 2010, Pages 124-136
نویسندگان
Fan-Jiang Kong, Atsushi Oyanagi, Setsuko Komatsu,