Hydrogen-exchange mass spectrometry for the study of intrinsic disorder in proteins

► HXMS is useful for discovery of intrinisically disordered (ID) regions in proteins. ► ID discovery and removal facilitates structure determination. ► ID discovery facilitates biopharmaceutical quality control. ► HXMS reveals coupled folding and binding of IDPs. ► HXMS reveals the regions of IDPs involved in aggregation.