کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10537083 | 962666 | 2009 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
α-Synuclein enhances bioluminescent activity of firefly luciferase by facilitating luciferin localization
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کلمات کلیدی
DTTCOABSA - BSAα-synuclein - α-سینوکلینATP assay - آزمون ATPbovine serum albumin - آلبومین سرم گاوEDTA - اتیلن دی آمین تترا استیک اسید Ethylenediaminetetraacetic acid - اتیلینیدامین تتراستیک اسیدParkinson's disease - بیماری پارکینسونBioluminescence - بیولومینسانسdithiothreitol - دیتیوتریتولFirefly luciferase - لوسیفراز فیرفیلیLuciferin - لوسیفرینcoenzyme A - کوآنزیم A
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
α-Synuclein, the pathological component of Parkinson's disease, has been demonstrated to be highly interactive with various protein partners. α-Synuclein has been shown to exert a novel effect on the bioluminescence of firefly luciferase by stimulating the oxyluciferin formation from its substrate of luciferin, which results in a significant enhancement of the spike of flashing light via concomitant augmentation for both rapid rise and quick decay of the luminescence. Binding affinity between α-synuclein and luciferase was evaluated with Kd of 8.1 µM based on a dose-dependent enhancement of the luciferase activity by α-synuclein. Kinetic analyses indicated that α-synuclein has facilitated luciferin localization to the luciferase by decreasing apparent Km, which makes the maximum rate of bioluminescence no longer dependent upon ATP concentration. Catalytic consequences of the α-synuclein binding to luciferase have led to a delayed onset of the coenzyme A-mediated retardation of the quick decay of flashing light as well as a shift in the emission spectra of bioluminescence. Taken together, the novel effects of α-synuclein toward the bioluminescence of luciferase have been demonstrated to be initiated by the specific molecular interaction between the proteins which has influenced the substrate (luciferin) localization to the enzyme.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics - Volume 1794, Issue 2, February 2009, Pages 309-314
Journal: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics - Volume 1794, Issue 2, February 2009, Pages 309-314
نویسندگان
Jehoon Kim, Chung Hee Moon, Seunho Jung, Seung R. Paik,