کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10537341 | 962716 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A common docking site for response regulators on the yeast phosphorelay protein YPD1
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کلمات کلیدی
hptCPRGHistidine-containing phosphotransferTwo-component signal transduction - انتقال سیگنال دو جزءProtein–protein interaction - تعامل پروتئین-پروتئینOsmoregulation - تنظیم فشار اسمزیResponse regulator - تنظیم کننده پاسخYeast two-hybrid assay - دوزهای هیبرید مخمرpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازmitogen-activated protein kinase - پروتئین کیناز فعال با mitogenMAP kinase - کیناز MAP
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A common docking site for response regulators on the yeast phosphorelay protein YPD1 A common docking site for response regulators on the yeast phosphorelay protein YPD1](/preview/png/10537341.png)
چکیده انگلیسی
In Saccharomyces cerevisiae, a multi-component phosphorelay signal transduction pathway mediates cellular responses to environmental stress. A histidine-containing phosphotransfer protein, YPD1, represents a bifurcation point between the SLN1-YPD1-SSK1 pathway responsible for osmotic stress responses and the SLN1-YPD1-SKN7 pathway involved in cell wall biosynthesis and cell cycle control. The phosphorelay protein YPD1 must physically interact with and transfer phosphoryl groups between three homologous response regulator domains, designated SLN1-R1, SSK1-R2, and SKN7-R3. In this comparative study, the molecular basis of interaction was examined between YPD1 and each of the three response regulator domains utilizing alanine scanning mutagenesis combined with a yeast two-hybrid assay. Results from the yeast two-hybrid assay indicate that all three response regulator domains bind to a common area, largely hydrophobic in nature, on the surface of YPD1. We postulate that other YPD1 surface residues surrounding this common docking site are involved in making specific interactions with one or more of the response regulator domains.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics - Volume 1748, Issue 2, 15 May 2005, Pages 138-145
Journal: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics - Volume 1748, Issue 2, 15 May 2005, Pages 138-145
نویسندگان
Stace W. Porter, Ann H. West,