Aggregation and structural changes of silver carp actomyosin as affected by mild acidification with d-gluconic acid δ-lactone

► Acid-induced aggregation and structural changes of actomyosin were investigated. ► Secondary and tertiary structure of actomyosin changed rapidly under acidic conditions. ► Aggregation occurred prior to completion of denaturation process during acidification. ► Myosin heavy chain was the major protein involved in non-covalent cross-linking and acid-induced aggregation.