In situ thermal denaturation of myofibre sub-type proteins studied by immunohistofluorescence and synchrotron radiation FT-IR microspectroscopy

► Thermal denaturation of muscle proteins is characterised by FT-IR microspectroscopy. ► Connective tissue is less affected by temperature than myofibrillar proteins. ► Myofibrillar proteins denaturation is weakly dependent on fibre sub-type.