Bioactivity evaluations of ingredients extracted from the flowers of Citrus aurantium L. var. amara Engl

► HTF and LA were separated from C. aurantium var amara for the first time. ► HTF had higher activity than 5-F on inhibiting proliferation of three cell lines. ► LA had a higher inhibition effect on MDA relative to Vc. ► HTF and LA have evident anti-inflammatory activity from 6.25 to 100 μg/ml.