Development and validation of an HPLC-method for determination of free and bound phenolic acids in cereals after solid-phase extraction

► A simple and reliable HPLC-DAD method for the determination of phenolic acids was developed. ► An optimised SPE was applied for the isolation of free phenolic acids from cereal samples. ► Bound phenolic acids were extracted using alkaline hydrolysis. ► Method validation was performed by means of linearity, intra-day accuracy, inter-day precision and sensitivity. ► The method was applied in durum and bread wheat, barley, oat, rice, rye, corn and triticale.