Immunoassay based on monoclonal antibody for aflatoxin detection in poultry feed

► An immunoassay was standardised and validated for aflatoxin screening in poultry feed. ► Its performance was compared to high-performance liquid chromatography. ► The immunoassay showed good linearity, low detection limit and high recovery rates. ► The immunoassay showed reliability and a high correlation with HPLC. ► The results indicated its potential for aflatoxin screening in poultry feed samples.