A new acetonitrile-free mobile phase for HPLC-DAD determination of individual anthocyanins in blackcurrant and strawberry fruits: A comparison and validation study

► The proposed acetonitrile-free method was suitable for routine quantification of berry anthocyanins. ► Methanol-based elution may be used as a fallback method if problems with acetonitrile supply persist/reoccur. ► Differences between individual anthocyanins in different strawberry and blackcurrant cultivars were elucidated.