Development of a fast MECK method for determination of 5-HMF in honey samples

► A MECK-UV method was developed using a full factorial for 5-HMF separation. ► Multiple regression enables the mathematical relationship for a response function. ► The analysis time is shorter than others CE methods reported in the literature. ► Under optimal CE conditions, 5-HMF peak was recorded in less than 0.7 min. ► Samples were dissolved in purified H2O and injected without any other pretreatment.