Indirect, non-competitive amperometric immunoassay for accurate quantification of calpastatin, a meat tenderness marker, in bovine muscle

► Highly sensitive amperometric immunoassay was developed for calpastatin detection. ► Generation of recombinant bovine calpastatin was described. ► Bovine skeletal muscle extract was used as real sample. ► Optimum sample dilution was found, for minimal interference effect. ► Accuracy of the method was confirmed by standard addition method.