Differentiation of 3-O-Sulfated Heparin Disaccharide Isomers: Identification of Structural Aspects of the Heparin CCL2 Binding Motif

CID was used to distinguish between heparin disaccharide isomers containing or lacking 3-O-sulfation and applied to evaluate the involvement of 3-O-sulfation in the heparin/CCL2 interaction.