کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10548090 | 965416 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
High-performance thin layer chromatography method for quantitative determination of four major anthraquinone derivatives in Rheum emodi
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
A high-performance thin layer chromatographic (HPTLC) method for the rapid and simple quantification of the four major anthraquinone derivatives i.e. physcion, chrysophanol, emodin and chrysophanol glycoside in Rheum emodi is described. HPTLC of anthraquinone derivatives was performed on pre-coated RP-18 F254S HPTLC plates. For achieving good separation, the mobile phase of methanol-water-formic acid (80:19:1, v/v/v) was used. The densitometric determination of anthraquinone derivatives was carried out at 445Â nm in reflection/absorption mode. The calibration curves were linear in the range of 20-100Â ng for physcion, 80-400Â ng for chrysophanol and emodin, and 200-1000Â ng for chrysophanol glycoside. The method was found to be reproducible and convenient for quantitative analysis of anthraquinone derivatives in the methanolic extract of rhizomes of R. emodi collected from three different locations of Western Himalaya, India.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1077, Issue 2, 10 June 2005, Pages 202-206
Journal: Journal of Chromatography A - Volume 1077, Issue 2, 10 June 2005, Pages 202-206
نویسندگان
Narendra P. Singh, Ajai P. Gupta, Arun K. Sinha, Paramvir S. Ahuja,