کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10548119 | 965422 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Determination of l-carnitine in food supplement formulations using ion-pair chromatography with indirect conductimetric detection
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Determination of l-carnitine in food supplement formulations using ion-pair chromatography with indirect conductimetric detection Determination of l-carnitine in food supplement formulations using ion-pair chromatography with indirect conductimetric detection](/preview/png/10548119.png)
چکیده انگلیسی
A novel method for the determination of l-carnitine in food supplement formulations was developed and validated, using ion-pair chromatography with indirect conductimetric detection. The chromatographic method was based on a non-polar (C18) column and an aqueous octanesulfonate (0.64 mM) eluent, acidified with trifluoroacetic acid (5.2 mM). The retention time was 5.4 min and the asymmetry factor 0.65. A linear calibration curve from 10 to 1000 μg/ml (r = 0.99998), with a detection limit of 2.7 μg/ml (25 μl injection volume), a repeatability %RSD of 0.8 (40 μg/ml, n = 5) and reproducibility %RSD of 2.6 were achieved. The proposed method was applied for the determination of carnitine in oral solutions and capsules. No interference from excipients was found and the only pretreatment step required was the appropriate dilution with the mobile phase. Recovery from spiked samples was ranged from 97.7 to 99.7% with a precision (%RSD, n = 3) of 0.01-2.1%.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1069, Issue 2, 1 April 2005, Pages 209-215
Journal: Journal of Chromatography A - Volume 1069, Issue 2, 1 April 2005, Pages 209-215
نویسندگان
Aikaterini Kakou, Nikolaos C. Megoulas, Michael A. Koupparis,