کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10550042 | 967012 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Analytical method development and validation of mianserin hydrochloride and its metabolite in human plasma by LC-MS
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Analytical method development and validation of mianserin hydrochloride and its metabolite in human plasma by LC-MS Analytical method development and validation of mianserin hydrochloride and its metabolite in human plasma by LC-MS](/preview/png/10550042.png)
چکیده انگلیسی
Mianserin is a tetracyclic antidepressant drug and administered as racemate of R (â) and S (+) mianserin hydrochloride in a dose of 30-90Â mg/day in divided doses. Liquid chromatography-mass spectroscopy (LC-MS) is a tool, which is widely used for determination of drug and their metabolites in biological fluids because of its high sensitivity and precision. Here we describe a liquid chromatography mass spectroscopy method for simultaneous determination of mianserin and its metabolite, N-desmethylmianserin, from human plasma using a liquid-liquid extraction with hexane:isoamylalcohol (98:2) and back extraction with 0.005Â M formic acid solution. This method is specific and linear over the concentration range of 1.00-60.00Â ng/ml for mianserin and 0.50-14.00Â ng/ml for N-desmethylmianserin in human plasma. The lowest limits of quantification (LLQ) is 1.00Â ng/ml for mianserin and 0.50Â ng/ml for N-desmethylmianserin. Intraday and interday precision (%C.V.) is <10% for both mianserin and N-desmethylmianserin. The accuracy ranges from 94.44 to 112.33% for mianserin and 91.85-100.13% for N-desmethylmianserin. The stability studies showed that mianserin and N-desmethylmianserin in human plasma are stable during short-term period for sample preparation and analysis. The method was used to assay mianserin and its metabolite, N-desmethylmianserin, in human plasma samples obtained from subjects who had been given an oral tablet of 30Â mg of mianserin.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 823, Issue 2, 5 September 2005, Pages 69-74
Journal: Journal of Chromatography B - Volume 823, Issue 2, 5 September 2005, Pages 69-74
نویسندگان
Bhupendrasinh Chauhan, Shubha Rani, Swati Guttikar, Anjali Zope, Nimisha Jadon, Harish Padh,