کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10550086 | 967016 | 2005 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Determination of riboflavin by high-performance liquid chromatography with riboflavin-depleted urine as calibration and control matrix
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Determination of riboflavin by high-performance liquid chromatography with riboflavin-depleted urine as calibration and control matrix Determination of riboflavin by high-performance liquid chromatography with riboflavin-depleted urine as calibration and control matrix](/preview/png/10550086.png)
چکیده انگلیسی
A simple method, exposure to natural-light, was developed to remove riboflavin from urine to enhance its use as the biological matrix for the preparation of calibration and control samples. Riboflavin-depleted urine containing less than 1 ng/ml of riboflavin was used to validate a high-performance liquid chromatography with fluorescence detection method for the determination of urinary riboflavin. The linearity of the assay (r2 = 0.999) was acceptable over the range of 10-5000 ng/ml. The intra-assay and inter-assay CVs were 3.3% and 9%, respectively. Subsequent stability studies found that urine riboflavin was stable for up to 6 months at 4 or â20 °C.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 820, Issue 1, 5 June 2005, Pages 147-150
Journal: Journal of Chromatography B - Volume 820, Issue 1, 5 June 2005, Pages 147-150
نویسندگان
Meng Chen, David M. Andrenyak, David E. Moody, Rodger L. Foltz,