کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10550089 | 967019 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Verapamil quantification in human plasma by liquid chromatography coupled to tandem mass spectrometry
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Verapamil quantification in human plasma by liquid chromatography coupled to tandem mass spectrometry Verapamil quantification in human plasma by liquid chromatography coupled to tandem mass spectrometry](/preview/png/10550089.png)
چکیده انگلیسی
An analytical method based on liquid chromatography with positive ion electrospray ionization (ESI) coupled to tandem mass spectrometry detection (LC-MS/MS) was developed for the determination of Verapamil in human plasma using Metoprolol as the internal standard. The analyte and internal standard were extracted from the plasma samples by liquid-liquid extraction and chromatographed on a C8 analytical column. The mobile phase consisted of methanol-water (70:30; v/v)Â +Â 12Â mM formic acid. The method had a chromatographic total run time of 3.5Â min and was linear within the range 1.00-500Â ng/mL. Detection was carried out on a Micromass Quattro Ultima tandem mass spectrometer by multiple reaction monitoring (MRM). The intra-run imprecision was less than 5.1% calculated from the quality control (QC) samples, and 16.3% from the limit of quantification (LOQ). The accuracy determined from QC samples were between 92.9 and 103.1%, and 95.2 and 115.3% from LOQ. Concerning the inter-batch analysis, the imprecision was less than 5.8% and 17.3% from QC samples and LOQ, respectively. The accuracy varied between 98.2 and 100.8% from QC and it was 103.1% from LOQ. The protocol herein described was employed in a bioequivalence study of two tablet formulations of Verapamil.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 827, Issue 2, 5 December 2005, Pages 165-172
Journal: Journal of Chromatography B - Volume 827, Issue 2, 5 December 2005, Pages 165-172
نویسندگان
Ney Carter do C. Borges, Gustavo D. Mendes, Rafael E. Barrientos-Astigarraga, Paulo Galvinas, Celso H. Oliveira, Gilberto De Nucci,