کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10550519 | 967066 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Measurement of serum pralidoxime methylsulfate (Contrathion®) by high-performance liquid chromatography with electrochemical detection
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Pralidoxime methylsulfate (Contrathion®) is widely used to treat organophosphate poisoning. Despite animal and human studies, the usefulness of Contrathion® therapy remains a matter of debate. Therapeutic dosage regimens need to be clarified and availability of a reliable method for plasma pralidoxime quantification would be helpful in this process. We here describe a high-performance liquid chromatography technique with electrochemical detection to measure pralidoxime concentrations in human serum using guanosine as an internal standard. The assay was linear between 0.25 and 50 μg mLâ1 with a quantification limit of 0.2 μg mLâ1. The analytical precision was satisfactory, with variation coefficients lower 10%. This assay was applied to the analysis of a serum from an organophosphorate poisoned patient and treated by Contrathion® infusions (100 and 200 mg hâ1) after a loading dose (400 mg).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 814, Issue 1, 5 January 2005, Pages 149-154
Journal: Journal of Chromatography B - Volume 814, Issue 1, 5 January 2005, Pages 149-154
نویسندگان
Pascal Houzé, Stephen W. Borron, François Scherninski, Bernard Bousquet, Bernard Gourmel, Frédéric Baud,