کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10551196 967111 2005 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of Salvinorin A in body fluids by high performance liquid chromatography-atmospheric pressure chemical ionization
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Determination of Salvinorin A in body fluids by high performance liquid chromatography-atmospheric pressure chemical ionization
چکیده انگلیسی
Salvinorin A was quantitated in human and rhesus monkey plasma, rhesus monkey cerebrospinal fluid, and human urine by negative ion LC-MS/APCI. The method for Salvinorin A has been fully validated, the LLOQ using FDA guidelines is 2 ng/mL for 0.5 mL plasma samples. The linear range was from 2 to 1000 ng/mL. Several derivatives in the Salvinorin family can also be analyzed by this method; d3-Salvinorin A was prepared and used as internal standard. The metabolite Salvinorin B can be semi quantitatively determined. The method has been used to establish that Salvinorin B is the principal metabolite of Salvinorin A ex vivo and to establish the analytical method to study in vivo samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 818, Issue 2, 25 April 2005, Pages 221-225
نویسندگان
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