کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10551317 | 967117 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Felodipine quantification in human plasma by high-performance liquid chromatography coupled to tandem mass spectrometry
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A rapid, sensitive, robust and specific method was developed for the determination and quantitation of felodipine, in human blood plasma by liquid chromatography coupled with tandem mass spectrometry using nimodipine as internal standard. Felodipine was extracted from 0.5 mL human plasma by use of a liquid/liquid procedure using diethyl ether/hexane (80/20, v/v) as eluent. The method included a chromatographic run of 5 min using a C18 analytical column (100 mm Ã 4.6 mm i.d.) and the calibration curve was linear over the range from 0.02 to 10 ng mLâ1 (r2 > 0.994). The between-run precision, determined as relative standard deviation of replicate quality controls, was 5.7% (0.06 ng mLâ1), 7.1% (0.6 ng mLâ1) and 6.8% (7.5 ng mLâ1). The between-run accuracy was ± 0.0, 2.1 and 3.1% for the above-mentioned concentrations, respectively.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 814, Issue 2, 25 January 2005, Pages 217-223
Journal: Journal of Chromatography B - Volume 814, Issue 2, 25 January 2005, Pages 217-223
نویسندگان
Luis H. Migliorança, Rafael E. Barrientos-Astigarraga, B.S. Schug, H.H. Blume, Alberto S. Pereira, Gilberto De Nucci,