کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
105522 161515 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Molecular epidemiology of vancomycin–resistant enterococci in Singapore
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی پزشکی قانونی
پیش نمایش صفحه اول مقاله
Molecular epidemiology of vancomycin–resistant enterococci in Singapore
چکیده انگلیسی

SummaryAimsTo characterise the mechanism of glycopeptide resistance, genetic relatedness, and pathogenicity factors in isolates of vancomycin-resistant enterococci (VRE) in Singapore.MethodsA total of 292 Enterococcus faecium and 17 Enterococcus faecalis were isolated from humans, and five E. faecium, two Enterococcus durans, two Enterococcus flavescens, one Enterococcus casseliflavus, and one Enterococcus gallinarum from chickens. The mechanism of glycopeptide resistance and pathogenicity factors were studied by PCR and the genetic relatedness determined by pulsed-field gel electrophoresis (PFGE), multi-locus variable number tandem repeat analysis (MLVA), and Tn1546 analysis.ResultsThere were five outbreak clones among the vancomycin-resistant E. faecium with one clone predominant. Four of the clones were vanB positive, and only one clone carried vanA. All outbreak clones were esp gene positive. Sporadic human isolates and chicken isolates were vanA positive and did not contain any pathogenicity genes. The situation was reversed in vancomycin-resistant E. faecalis where almost all isolates were vanA positive.ConclusionsMost VRE in Singapore is hospital associated with a small number of clones of esp-positive vanB E. faecium responsible for the majority of isolates.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Pathology - Volume 41, Issue 7, December 2009, Pages 676-680