کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10553011 | 967666 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Solid phase extraction-gas-chromatographic method to determine free cholesterol in animal fats
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
An analytical method to determine cholesterol in animal fats was developed. Cholesterol of four different animal fats was analysed by solid phase extraction, using porous silica derivatized with NH2 groups. The compound, purified from glycerides, was quantified as its trimethylsilyl derivative by gas chromatography coupled with a flame ionization detector. The results obtained with the proposed method were compared with those obtained with a saponification method. Proposed method repeatability and accuracy were reasonable. Coefficient of variation relative to standard deviation (s.d.) of repeatability was ⩽3.5%, while recovery was on average 96.2%. The limit of detection was about 0.8 μg of cholesterol per mg of sample loaded, with a s.d. of 0.01 μg. In conclusion, the proposed method is simple, fast and reproducible.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Food Composition and Analysis - Volume 18, Issue 7, November 2005, Pages 617-624
Journal: Journal of Food Composition and Analysis - Volume 18, Issue 7, November 2005, Pages 617-624
نویسندگان
Mario Vincenzo Russo, Antonella De Leonardis, Vincenzo Macciola,