Nicotinamide and resveratrol regulate bovine adipogenesis through a SIRT1-dependent mechanism

The activity of SIRT1 is regulated by many chemical compounds, such as nicotinamide and resveratrol. Here, we probed the specific functions of nicotinamide and resveratrol on bovine adipocyte proliferation and differentiation and showed that inhibition of SIRT1 by nicotinamide increased the number of preadipocytes and expression of adipocyte markers. Resveratrol-induced SIRT1 activation significantly repressed proliferation of preadipocyte, and lessened the expression levels of adipogenic gene. By using an shRNA approach, nicotinamide and resveratrol were further shown to modulate bovine adipogenesis through a SIRT1-dependent mechanism because there were no fat changes responding to drug treatment in SIRT1 knockdown adipocytes. Taken together, the data in this work not only enhanced the theory that resveratrol has pronounced anti-obesity effects in adipose tissue but also indicated that nicotinamide has a positive impact on bovine adipogenesis, and orally administered nicotinamide may be a potential means to promote bovine adipogenesis.