کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10553579 | 967877 | 2011 | 17 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Chiral separations in normal-phase liquid chromatography: Enantioselectivity of recently commercialized polysaccharide-based selectors. Part II. Optimization of enantioselectivity
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Earlier, a set of pharmaceuticals with different chemical structures has been used to evaluate the enantioselectivity of four recently commercialized polysaccharide-based chiral stationary phases, Lux® Cellulose-1/Sepapak® 1, Lux® Cellulose-2/Sepapak® 2, Lux® Amylose-2/Sepapak® 3 and Lux Cellulose-4/Sepapak® 4 and of three Daicel columns, Chiralpak® AD-H, Chiralcel® OD-H and Chiralcel® OJ-H, using the screening conditions of an existing generic separation strategy in normal-phase liquid chromatography (NPLC). In this study, the applicability of the optimization steps of the existing separation strategy was examined using 44 drugs (70 optimization cases) representing the three possible resolution situations that occur after screening. Optimizations are demonstrated by modifying parameters such as polar modifier percentages, temperatures, flow rates and additives concentration. Changing the percentage of polar modifier was found to have the largest influence on the resolution. The resolution, peak shape and the analysis time were nicely improved for 49/70 cases (70%) after the application of the original optimization steps. The introduction of some modifications to the original optimization increased this number from 49 to 62 cases, i.e. from 70% to 88.6%. Finally, an updated generic separation strategy in NPLC was proposed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 56, Issue 3, 1 November 2011, Pages 521-537
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 56, Issue 3, 1 November 2011, Pages 521-537
نویسندگان
Ahmed A. Younes, Debby Mangelings, Yvan Vander Heyden,