کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10553597 | 967877 | 2011 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development and validation of an ion-pair RP-HPLC method for the determination of oligopeptide-20 in cosmeceuticals
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Oligopeptide-20 is a growth-factor mimicking peptide used in cosmeceuticals. This article describes the development and validation of an ion-pair reversed-phase liquid chromatography method that allows, after liquid-liquid extraction, the quantification of oligopeptide-20 in cosmetic creams. Chromatographic separation was achieved on a cyanopropyl Hypersil analytical column (100 mm Ã 2.1 mm i.d., 5 μm particle size), using a mobile phase of acetonitrile-heptafluorobutyric acid (pH = 2.5, 9.0 mM) (70:30, v/v) containing 0.045% diethylamine at a flow rate of 0.50 mL minâ1. Ultraviolet (UV) spectrophotometric detection at 225 nm was used. The method had linear calibration curve over the range 1.35-4.95 μg mLâ1 for oligopeptide-20. The intra- and inter-day RSD values were less than 3.3%, while the relative percentage error, %Er, was less than 1.9. The developed method was applied successfully to the quality control of a cosmetic cream containing 0.003% (w/w) oligopeptide-20.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 56, Issue 3, 1 November 2011, Pages 645-649
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 56, Issue 3, 1 November 2011, Pages 645-649
نویسندگان
Panagiota Papagianni, Athanasia Varvaresou, Spyros Papageorgiou, Irene Panderi,