کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10553688 | 967909 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Electrochemical genosensing of the interaction between the potential chemotherapeutic agent, cis-bis(3-aminoflavone)dichloroplatinum(II) and DNA in comparison with cis-DDP
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
The interaction of cis-diamminedichloroplatinum(II) (cis-DDP) and the potential novel chemotherapeutic agent, cis-bis(3-aminoflavone)dichloroplatinum(II) (cis-BAFDP) was studied electrochemically with calf thymus double-stranded DNA (dsDNA) by using differential pulse voltammetry (DPV) with disposable pencil graphite electrode (PGE) at the surface. These studies were prompted by beneficial biological properties of cis-BAFDP in comparison with cis-DDP, which were proven in vitro both in human normal and cancer cells and in vivo. The changes in the experimental parameters such as the concentration of cis-DDP and cis-BAFDP were studied by using DPV; in addition, the reproducibility of this genosensor and the detection limit for each compound were determined. After the interaction of cis-DDP with dsDNA, the DPV signal of guanine and adenine was found to be decreasing. In comparison with cis-DDP, a dramatic decrease at adenine signal was also obtained after the interaction of cis-BAFDP and dsDNA. Similar results were also found in solution phase after the latter compound interacts with poly[A]. The features of the proposed electrochemical method for the detection of cis-BAFDP with DNA in comparison with cis-DDP are discussed and compared with those methods previously reported for the other type of DNA-targeted agents in the literature.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 38, Issue 4, 15 July 2005, Pages 645-652
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 38, Issue 4, 15 July 2005, Pages 645-652
نویسندگان
Arzum Erdem, Beata Kosmider, Regina Osiecka, Elzbieta Zyner, Justyn Ochocki, Mehmet Ozsoz,