کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10553998 | 967947 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Chemical fingerprinting of Isatis indigotica root by RP-HPLC and hierarchical clustering analysis
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Chemical fingerprinting of Isatis indigotica root by RP-HPLC and hierarchical clustering analysis Chemical fingerprinting of Isatis indigotica root by RP-HPLC and hierarchical clustering analysis](/preview/png/10553998.png)
چکیده انگلیسی
The aim was to establish a method for extraction and chemical fingerprinting of extracts of Isatis indigotica roots (“Ban-Lan-Gen”) and to apply the method developed to 18 Ban-Lan-Gen samples. RP-HPLC with gradient elution was performed on authentic reference standards of powdered I. indigotica roots, indigotin and indirubin purchased from the National Institute for the Control of Pharmaceutical and Biological Products (NICPBP) of China. Eighteen “Ban-Lan-Gen” samples (including the reference powdered herb) were bought from Singapore and different regions in China. Comparisons of the chromatograms showed that the samples can be divided into three groups. The chromatograms of the extracts of five samples were found to be similar to that of the extract of the authentic sample. Eight other samples had similar peaks as the authentic sample but the intensities of the peaks were generally lower, except for the peaks between retention times of 10-40Â min. Peaks in these regions were more intense than those found in the extract of the authentic sample. Forty-five characteristic peaks could be found in the extracts of all the above samples. Peaks at retention times 52 and 53Â min were determined to be indigotin and indirubin, respectively. The remaining four samples had similar chemical fingerprints to each other but were different from that of the authentic sample. Hierarchical clustering analysis gave similar results as the visual comparison. The RP-HPLC method developed allows simple identification and comparisons of I. indigotica roots. This is the first report of hierarchical clustering analysis of I. indigotica root.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 38, Issue 3, 1 July 2005, Pages 514-520
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 38, Issue 3, 1 July 2005, Pages 514-520
نویسندگان
P. Zou, Y. Hong, H.L. Koh,