کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10554007 967947 2005 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Simultaneous determinations of paeonol and palmatine hydrochloride in Shangshi Aerosols by HPLC method
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Simultaneous determinations of paeonol and palmatine hydrochloride in Shangshi Aerosols by HPLC method
چکیده انگلیسی
A simple and rapid HPLC method was described for the simultaneous determination of paeonol and palmatine hydrochloride in Shangshi Aerosols. The optimum separation for these analytes was achieved using the mixture of 0.025 M sodium dihydrogen phosphate-acetonitrile-diethylamine (64:35:1, v/v/v) as the mobile phase and a Nova-Pak® C8 column. The linear ranges of paeonol and palmatine hydrochloride were 0.2-80 and 0.06-60 μg/ml with the regression equations being Y = 11716.4 + 2.96 × 106X (R = 0.99969), Y = −6388.8 + 1.89 × 105X (R = 0.99976), and limit of quantifications (LOQ) for paeonol and palmatine hydrochloride were 0.2 and 0.06 μg/ml, respectively (n = 6). Other validation parameters: intra-day precision (R.S.D.: 0.71-1.65%) and inter-day precision (R.S.D.: 0.89-2.11%), and reproducibility (recoveries values: 94.6-98.2% for paeonol, 94.85-97.58% for palmatine hydrochloride) were found to be satisfactory. The proposed HPLC method had been applied for the determination of paeonol and palmatine hydrochloride in Shangshi Aerosols; R.S.D. values were 1.45 and 1.13%, respectively. In short, this method was rapid and convenient, which could be used for the routine control of paeonol and palmatine hydrochloride in Shangshi Aerosols.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 38, Issue 3, 1 July 2005, Pages 571-575
نویسندگان
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