کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10554032 | 967949 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Determination of stability constant values of flurbiprofen-cyclodextrin complexes using different techniques
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Three new experimental approaches for calculating the stability constant (Kst) of complexes of flurbiprofen with natural β-cyclodextrin (βCyd) and the hydroxyethyl- (HEβCyd) and the methyl- (MeβCyd) derivatives were tested and compared to the classic phase-solubility procedure: (a) the membrane permeation technique through a lipophilic synthetic membrane permeable to the drug but not to the Cyd molecules, by analysing the permeation profiles with a non-linear least-squares method; (b) the affinity capillary electrophoresis (ACE) technique, where Kst were calculated from the relationship between Cyd concentration in solution and drug electrophoretic mobility, using three different linear plotting methods; (c) the molecular modeling technique, based on the relationship between the docking energies and the experimental Kst values. The study allowed evaluation of the advantages and limits of each examined method, providing a useful guide for the choice of the most suitable one depending on the kind of host-guest system to be investigated. The Kst values obtained with the various techniques were rather different, probably due to the very different experimental conditions required by each one. However, all the methods indicated the methyl-derivative as the most powerful complexing agent for the drug, showing the general trend: Kst(MeβCyd) >> Kst(HEβCyd) > Kst(βCyd). Only in the case of the ACE method was an inversion of the trend found between HEβCyd and βCyd; this was probably due to the lower molecular weight of the natural Cyd, which, in this case, became more important in determining the complex electrophoretic mobility than the different affinity of the drug for these two Cyds.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 37, Issue 5, 29 April 2005, Pages 995-1002
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 37, Issue 5, 29 April 2005, Pages 995-1002
نویسندگان
M. Cirri, F. Maestrelli, S. Orlandini, S. Furlanetto, S. Pinzauti, P. Mura,