کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10554362 | 967959 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Electrochemical detection of enzyme labeled DNA based on disposable pencil graphite electrode
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
Electrochemical biosensor for the detection of DNA hybridization using the reduction signal of α-naphthol is described. A pencil graphite electrode was used as a working electrode. Capture probes were covalently attached on to the pencil graphite electrode surface (PGE) at the 5â² end amino group by using N-(dimethylamino)propyl-Nâ²-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysulfosuccinimide (NHS) as a coupling agent on to PGE. After capture probe immobilization on to PGE surface; probe was hybridized with complementary biotinylated oligonucleotide. Alkaline phosphatase labeled with extravidin (Ex-AP) binds to biotinylated hybrid via biotin-avidin interaction. α-Naphthyl phosphate (α-NAP) was added and the reaction between alkaline phosphatase (AP) and α-NAP was occurred consequently as a substrate of AP, α-NAP reduction signal was obtained from this reaction, at â0.100 V by using differential pulse voltammetry (DPV). Other experimental parameters were studied such as; optimizations of hybridization time, and the concentrations of capture probe, biotinylated oligonucleotide and enzyme.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 38, Issue 1, 1 June 2005, Pages 191-195
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 38, Issue 1, 1 June 2005, Pages 191-195
نویسندگان
Pinar Kara, Arzum Erdem, Stella Girousi, Mehmet Ozsoz,