کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10554469 | 967963 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Simultaneous determination of ranitidine and metronidazole in human plasma using high performance liquid chromatography with diode array detection
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Simultaneous determination of ranitidine and metronidazole in human plasma using high performance liquid chromatography with diode array detection Simultaneous determination of ranitidine and metronidazole in human plasma using high performance liquid chromatography with diode array detection](/preview/png/10554469.png)
چکیده انگلیسی
The development and validation of a simple method for the simultaneous determination of ranitidine and metronidazole in human plasma is described. Plasma samples (250 μL) were deproteinized by precipitation with 60% perchloric acid, centrifuged and the supernatant directly injected into the HPLC. Separation was achieved in isocratic mode with a Shimpak C18 column and a mobile phase consisting of 10 mM potassium dihydrogen phosphate pH 3.5:acetonitrile (90:10, v/v) with UV detection at 315 nm. The method showed good selectivity and sensitivity. Good and consistent recovery for metronidazole and ranitidine was obtained: 96.22 ± 3.52 and 95.00 ± 4.50% for ranitidine (25-1000 ng/mL) and metronidazole (60-10,000 ng/mL), respectively (n = 3). With this one-step sample preparation method, both ranitidine and metronidazole could be quantified simultaneously in human plasma with good precision (R.S.D. < 15%) and accuracy (bias values below 15%). The limit of quantification for ranitidine and metronidazole were 20 and 40 ng/mL plasma, respectively.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 37, Issue 4, 1 April 2005, Pages 777-783
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 37, Issue 4, 1 April 2005, Pages 777-783
نویسندگان
Ticiano Gomes do Nascimento, Eduardo de Jesus Oliveira, Rui Oliveira Macêdo,