Combined snake venomics and venom gland transcriptomic analysis of Bothropoides pauloensis

► The repertoire of venom toxins of B. pauloensis was assessed by venomic and transcriptomic surveys. ► Both approaches converge in their overall major toxin family profile but depart in the occurrence of minor components. ► The toxin profile explains the local and systemic effects observed in envenomations by B. pauloensis. ► B. pauloensis expresses the adult venom phenotype described in other Bothrops species.