Docosohaexanoic acid-supplemented PACA44 cell lines and over-activation of Krebs cycle: An integrated proteomic, metabolomic and interactomic overview

► We confirmed that DHA-supplementation triggered the onset of apoptosis in PACA44 cell lines at 48 to 72h from the treatment. ► We performed differential proteomics analyses which yielded identification of 26 differentially-expressed proteins. ► In silico analyses highlighted that these proteins were mainly involved in structural changes and metabolism modulation. ► MS-based metabolomics evidenced a switch towards the pentose phosphate pathway and Krebs cycle. ► DHA-triggered apoptosis in PACA44 cells might be related to the metabolic modulation.