Development of quantitative plasma N-glycoproteomics using label-free 2-D LC-MALDI MS and its applicability for biomarker discovery in hepatocellular carcinoma

► Development of new N-glycoproteomic method using label-free 2D-LC-MALDI. ► This minor procedure has a good performance for analyzing and quantifying plasma proteins. ► In a feasibility study, finding plasma biomarker candidates for hepatocelluler carcinoma verify the practicability of the new method.