Thiol redox proteomics seen with fluorescent eyes: The detection of cysteine oxidative modifications by fluorescence derivatization and 2-DE

► Reversible protein cysteine oxidation is now a key issue in cell signalling. ► Fluorescence derivatization coupled to 2DE allows to study these modifications. ► We critically review methodological differences in the techniques used. ► They have allowed to study protein Cys oxidation in some physiological scenarios.