Quantitative in-depth analysis of the dynamic secretome of activated Jurkat T-cells

► A new technology for the systematic high-throughput quantitative study of dynamic secretomes. ► The deepest quantitative analysis to date of proteins secreted by lymphoid Jurkat T-cells upon activation. ► A robust protocol for high-throughput analysis of proteomes by 18O labeling. ► Data analysis on the bases of a statistical model with a validated null hypothesis.