A differential proteomic approach identifies structural and functional components that contribute to the differentiation of brain capillary endothelial cells

► From an in vitro differential model of blood-brain barrier (BBB). ► A 2-DE approach to detect the differences in the Triton-X-100-solubilized proteins. ► Quantitative changes affect proteins of (i) cell structure and motility and (ii) of protein metabolism. ► Interesting fold-changes were confirmed by WB without mRNA expression level change ► The actin cytoskeleton reorganization contributes fully to the BBB phenotype.