“Proteomineering” or not? The debate on biomarker discovery in sera continues

► Thou shalt not add 150 mM salt to the sample. ► Thou shalt capture at three pH values. ► Thou shalt not elute in 4 M urea, 1% CHAPS. ► Thou shalt not elute in 8 M urea, 2% CHAPS, 5% acetic acid. ► Thou shalt elute in boiling 4% SDS, 25 mM DTT (A Pentalogue found in proximity of Mount Sinai).