The use of a novel quantitation strategy based on Reductive Isotopic Di-Ethylation (RIDE) to evaluate the effect of glufosinate on the unicellular algae Ostreococcus tauri

► We present a new labeling strategy for quantitative proteomics. ► The labeling is based on diethylation and can be done with 12C/13C or 1H/2H for mass pairing. ► The proposed labeling is universal, safer than other alternatives + easy to analyze. ► Labeling strategy tested on unicellular algae Ostreococcus tauri with herbicide (glufosinate). ► With herbicide, nitrogen assimilation goes down; oxidative stress + lipid metabolism go up.