کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10556261 | 968269 | 2011 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Saccharomyces cerevisiae in the stationary phase as a model organism - characterization at cellular and proteome level
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
⺠S. cerevisiae in the stationary phase was characterized at the cellular and proteome level. ⺠At the cellular level, optical density, cell viability, glycogen content, intracellular oxidation and cell energy metabolic activity were measured, while at the proteome level, protein profiles were analyzed using two-dimensional electrophoresis. ⺠The data obtained at both levels provide better insight into quiescence program state, which still remains poorly understood. ⺠Optimal time period reflecting a stable metabolic and oxidative state of the yeast was determined (2 to 8 days in PBS buffer-plateau 1). ⺠As such, this yeast system shows potential for studying changes in cell oxidant status as well as for energy metabolic activity in response to different environmental stressors.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Proteomics - Volume 74, Issue 12, 18 November 2011, Pages 2837-2845
Journal: Journal of Proteomics - Volume 74, Issue 12, 18 November 2011, Pages 2837-2845
نویسندگان
Teja Zakrajšek, Peter Raspor, Polona Jamnik,