Identification and quantification of concentration-dependent biomarkers in MCF-7/BOS cells exposed to 17β-estradiol by 2-D DIGE and label-free proteomics

► MCF-7/BOS exposure to 17β-estradiol and xenoestrogens. ► 2-D DIGE/MALDI TOF MS and label-free 2-D UPLC MSE on cytosolic fractions. ► Identification of concentration-dependent protein expression signature. ► Biomarkers in close association with hormonal-dependent cellular response. ► Biomarkers used for SRM targeted proteomics for food chain control.