Filter-aided sample preparation with dimethyl labeling to identify and quantify milk fat globule membrane proteins

► FASP and dimethyl labeling were applied to identify and quantify milk fat globule membrane (MFGM) proteins. ► 169 proteins were identified in MFGM. ► 139 proteins were quantified in MFGM. ► FASP combined with dimethyl labeling is accurate and easy for MFGM protein quantification.