Shotgun proteome analysis of beer and the immunogenic potential of beer polypeptides

► The shotgun proteomic analysis extended the characterization of the beer proteome. ► Intact hordeins occur in beer, although in trace amounts. ► Beer contains hordeins that are immunoreactive to celiacs' sera. ► Beer peptides encrypt gluten-like motifs that are harmful to celiacs. ► tTGase-deamidated beer polypeptides activate a response in celiacs' gut T-cells.