کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10559510 | 969627 | 2011 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Centrifuge microextraction coupled with sweeping-MEKC to analyze trace steroid hormones in urine samples
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
A novel method that combines two concentration techniques, off-line centrifuge microextraction (CME) and on-line sweeping, is used to determine trace steroids in urine by micellar electrokinetic chromatography (MEKC). The CME-sweeping-MEKC is a promising technique, which has a variety of merits such as high sensitivity, rapid operation, minimal cost, and high sample throughput capability. Using CME-sweeping, over a 500-fold increase in sensitivity could be obtained as compared with the normal hydrodynamic injection without sample stacking. The linear range was 0.05-1 μg mLâ1 with the square of the correlation coefficients ranging from 0.9998 to 0.9999. Detection limits (S/N = 3) were 5-15 ng mLâ1 using a photodiode array UV detection at a wavelength of 240 nm. The relative standard deviations were between 2.8% and 3.5% (n = 5, progesterone as the internal standard). The diode array UV spectrum used can distinguish the analytes from the interference in the complex sample matrix, which is useful in biological and clinical sample analysis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 85, Issue 4, 30 September 2011, Pages 2148-2153
Journal: Talanta - Volume 85, Issue 4, 30 September 2011, Pages 2148-2153
نویسندگان
Huaifang Fang, Fangxing Yang, Junling Sun, Yun Tian, Zhaorui Zeng, Ying Xu,