کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10559510 969627 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Centrifuge microextraction coupled with sweeping-MEKC to analyze trace steroid hormones in urine samples
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Centrifuge microextraction coupled with sweeping-MEKC to analyze trace steroid hormones in urine samples
چکیده انگلیسی
A novel method that combines two concentration techniques, off-line centrifuge microextraction (CME) and on-line sweeping, is used to determine trace steroids in urine by micellar electrokinetic chromatography (MEKC). The CME-sweeping-MEKC is a promising technique, which has a variety of merits such as high sensitivity, rapid operation, minimal cost, and high sample throughput capability. Using CME-sweeping, over a 500-fold increase in sensitivity could be obtained as compared with the normal hydrodynamic injection without sample stacking. The linear range was 0.05-1 μg mL−1 with the square of the correlation coefficients ranging from 0.9998 to 0.9999. Detection limits (S/N = 3) were 5-15 ng mL−1 using a photodiode array UV detection at a wavelength of 240 nm. The relative standard deviations were between 2.8% and 3.5% (n = 5, progesterone as the internal standard). The diode array UV spectrum used can distinguish the analytes from the interference in the complex sample matrix, which is useful in biological and clinical sample analysis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 85, Issue 4, 30 September 2011, Pages 2148-2153
نویسندگان
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