Preparation of a strong-cation exchange monolith by a novel method and its application in the separation of IgG on high performance liquid chromatography

► Strong cation exchange monolith was prepared by ATRP. ► The ATRP process was realized without complexing ligand. ► The novel technique led a more uniform structure. ► The monolith was used to separate IgG and Lys with good resolution. ► The dynamic binding capacity of the monolith was much higher than that of the previous.