A reagent-free method based on a photo-induced fluorimetry in a sequential injection system

The calibration graph for indomethacin was linear between 4.10 × 10−6 and 9.00 × 10−5 mol L−1and the detection limit was 1.23 × 10−6 mol L−1. The method was proven to be reproducible with a R.S.D. < 5% and sampling rate of approximately 20 per hour. The potential effect of several compounds commonly used as excipients on analytical signals was studied and no interfering effect was observed. Statistical evaluation at the 95% confidence level showed good agreement between the results obtained for the pharmaceutical samples with both the SIA system and comparison batch procedures.