کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10559958 | 969698 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Enzyme/semiconductor nanoclusters combined systems for novel amperometric biosensors
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کلمات کلیدی
CTABSAMsPOHFDHNanoclustersNCSnicotinamide adenine dinucleotide (reduced form) - nikotinamide adenine dinucleotide (فرم کاهش یافته)W/O - W / Owater-in-oil - آب در روغنAmperometric biosensor - بیوسنسور آمپرترتریکSelf-assembled monolayers - تک لایه های خودآرا، تک لایه های خودسامانformaldehyde dehydrogenase - فرمالدئید دهیدروژنازLod - لودlimit of detection - محدودیت تشخیصNAD - نادانNADH - نادانCdS nanoparticles - نانوذرات CdSnicotinamide adenine dinucleotide - نیکوتین آمید adenine dinucleotidePentanol - پنتانولWorking electrode - کار الکترودGlutaraldehyde - گلوتارالدئید
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In this work quantum-sized CdS nanocrystals were synthesized using a quaternary water-in-oil microemulsion and immobilized onto gold working electrode by self-assembled monolayers techniques. Formaldehyde dehydrogenase was covalently immobilized onto a protecting membrane, which was stratified on part of the semiconductor nanoparticles modified electrode. The covalent enzyme immobilization has been required to improve the stability of the catalytic oxidation of formaldehyde, which occurs after light stimulation of the semiconductor through the electron/hole recombination. A study about the best electrochemical oxidation potentials under different flow conditions was performed. Preliminary sensor stability and interferences tests were also carried out, for a sensitive and selective detection of formaldehyde. A detection limit of 41Â ppb of formaldehyde was calculated and an operational stability of 6Â h was achieved under flow conditions by means of this novel amperometric biosensor based on FDH-semiconductor hybrid systems, not requiring NAD+/NADH as charge transfer in the enzymatic reaction.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 66, Issue 3, 30 April 2005, Pages 627-633
Journal: Talanta - Volume 66, Issue 3, 30 April 2005, Pages 627-633
نویسندگان
W. Vastarella, R. Nicastri,