کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10560147 | 969706 | 2011 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Solid-support fluorescent derivatization of picomoles of protein at low concentration with FITC
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Solid-support fluorescent derivatization of picomoles of protein at low concentration with FITC Solid-support fluorescent derivatization of picomoles of protein at low concentration with FITC](/preview/png/10560147.png)
چکیده انگلیسی
A new method based on solid-support reaction is described to realize fluorescent derivatization of proteins at concentrations as low as 10â8Â M. A simple, low-cost homemade capillary C18 cartridge was fabricated as the solid-support reactor. Using bovine serum albumin (BSA) as a test protein, we demonstrated that the protein can be captured by this reactor and then labeled by fluorescein isothiocyanate (FITC, isomer I) on solid-support. Unwanted fluorescent intruder (excrescent FITC and products of secondary reactions) were removed from target easily. The analysis by nano-HPLC with laser-induced fluorescence (LIF) detection was described. The effect of reaction conditions on the derivatization has been evaluated and discussed. The use of the solid-support reactor allows easy handling of as little as 8.5Â pmol of BSA. A fraction from weak anion-exchange chromatography (WAX) of human liver extract was used as an illustrative example of application to real samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 84, Issue 2, 15 April 2011, Pages 457-461
Journal: Talanta - Volume 84, Issue 2, 15 April 2011, Pages 457-461
نویسندگان
Qian Tao, Ming-Xia Gao, Guang-Feng Hong, Qi Chen, Xiang-Min Zhang,